As shown by LDH assay, 1 h pretreatment with necroptosis specific inhibitor Nec-1 (100 μmol/L), GSK-872 (20 μmol/L), and NSA (10 μmol/L) did not prevent silibinin-induced glioma cell death (Fig. S1I). The gene discussed is PCSK1; the disease is central nervous system cancer.