To evaluate whether the proliferation inhibitory effect of HHT on HCC cells was related to EphB4 expression, EphB4 siRNA or plasmid was utilized to transfect the HCC cells (Figs. 2a and S1b), and EphrinB2 Fc was used to stimulate the HCC cells. The gene discussed is EPHB4; the disease is hepatocellular carcinoma.