TTN-AS1 and cholangiocarcinoma: For examining the regulatory effects between TTN-AS1 and miR-320a, we first showed that transfection of miR-320a mimics had little effect on TTN-AS1 expression, but depletion of TTN-AS1 significantly increased the expression of miR-320a in RBE and HCCC9810 cells (Supplementary Fig. S4A, B), implying that TTN-AS1 might negatively regulate miR-320 in CCA cells.