We performed microvessel density formation assay and counted number of CD34-positive blood vessels on subcutaneous tumor tissue of nude mice, showing that knockdown of FBX8 increased the number of micro-angiogenesis in subcutaneous tumors and HUVEC cells lumen formation in vitro (Fig. 6a; P < 0.01, P < 0.001, Fig. 6b, c; P < 0.001, Fig. 6d). This evidence concerns the gene CD34 and neoplasm.