TP53 and melanoma: For example, the above-cited paper by Berndtsson et al. [69], which showed a clear apoptotic threshold at approximately 10 μM, concluded that, while biologically/clinically-relevant concentrations of cisplatin (i.e., ≤20 μM) did not induce apoptosis, they did cause p53-WT HCT116 colon carcinoma and 224 melanoma cells to undergo an extensive and sustained growth arrest that was associated with SA-β-gal activity and an approximately 3-fold increase in p53 levels.