Berndtsson et al. [69] confirmed the threshold nature of the apoptotic response in p53-WT HCT116 colon carcinoma and 224 melanoma cells exposed to cisplatin for 6 h, with significant levels of apoptosis only being seen after much higher cisplatin concentrations (threshold at approximately 10 μM) than those used in the CF assay, as shown in Figure 3. Here, TP53 is linked to melanoma.