Riedl et al. reported significant differences in mTOR activity and crosstalk between AKT-mTOR-S6K and the MAPK pathway in spheroids vs. planar cultures of colorectal cancer Caco-2 cells, including alterations in the responses in treatments with inhibitors of AKT, mTOR and S6K axis or of the MAPK (ERK) axis, which are ongoing pharmacological targets [15]. This evidence concerns the gene MTOR and colorectal cancer.