Conversely, subgroup 3 had normal expression of MHC class II, but low level of TMB and low expression of MHC class I molecules, which would result in rapid clearance of the clonal fraction of neoantigen-containing tumor cells by the resultant neoantigen-specific cytotoxic CD4 T cells, even in small number (Takeuchi and Saito, 2017), and subsequent conversion to memory resting cells with perforins and granzyme B still retained (Lin et al., 2014). Here, PRF1 is linked to neoplasm.