Western blotting analysis further confirmed the results that the expression levels of Gata4 and Nkx2–5 protein in the myocardium of the offspring in the SCH group was significantly lower than that in the Sham group, while L-T4 treatment further significantly increased the expression levels of Gata4 and Nkx2–5 protein in the myocardium of the offspring in the LT4-E10 and LT4-E13 groups (Fig. 5c-d, compared to SCH group, p < 0.05). The gene discussed is NKX2-5; the disease is spindle cell hemangioma.