Using 2D colonoid monolayers plated on permeable membrane scaffolds, it was reaffirmed that basolateral infection by S. flexneri is significantly more efficient than apical infection.21,22 Using differentiated colonoids containing mucus-producing goblet cells, Shigella infection was shown to cause increased production of the major intestinal mucin glycoprotein MUC2.22 The gene discussed is MUC2; the disease is infection.