To determine whether LRCs in the BM of mice with WT or Selplg–/– AML had a similar potential to survive chemotherapy and initiate relapse in vivo, we used a modified version of the limiting-dilution transplant assay typically used as gold-standard for quantifying functional HSC numbers (Purton and Scadden, 2007). The gene discussed is SELPLG; the disease is acute myeloid leukemia.