Moreover, the curve of p.E545K <i>PIK3CA</i> in EFIRM showed similarity to those of tumor volume (<i>R</i> = 0.70, <i>P</i> = 0.00) and p.E545K <i>PIK3CA</i> in NGS (<i>R</i> = 0.72, <i>P</i> = 0.00). Here, PIK3CA is linked to neoplasm.