As shown in Table 7, the average survival time of GBC patients (including SC/ASC and AC) was significantly related to several clinicopathological parameters, including tumor differentiation, tumor size, TNM stage, lymph node metastasis, invasion, receiving the surgical procedure, and XBP1 and NAT1 expression (all P < 0.05). This evidence concerns the gene NAT1 and metastatic malignant neoplasm in the lymph nodes.