To evaluate whether PBMCs are able to induce EMT in the epithelial-like or mesenchymal-like breast cancer cells, we co-cultured freshly isolated PBMCs with two human breast cancer cell lines (MCF-7 and MDA-MB-231) exhibiting two different patterns of E-cadherin and N-cadherin expression for 5 days. The gene discussed is CDH1; the disease is breast carcinoma.