Pathway analysis of the secretome from the co-cultured PBMCs vs. control PBMCs identified 44 significantly enriched pathways based on the P < 0.05, the most prominent of which were as follows: oocyte meiosis, cell cycle, glioma, prion diseases, starch and sucrose metabolism, thyroid cancer, bladder cancer, PI3K-Akt signaling pathway, human immunodeficiency virus 1 infection, human T-cell leukemia virus 1 infection, and HIF-1 signaling pathway. The gene discussed is AKT1; the disease is urinary bladder carcinoma.