In the present study, we found higher positive rates of autoantibodies against ALDH1B1, UQCRC1, CTAG1, and CENPF with positive rates of 62.7, 54.2, 64.4, and 62.7%, respectively, in early CRC compared with that of 38.6% for CEA, and more than half of the early CRC cases negative for CEA were positive for these TAAbs, implying the potential value of these TAAbs for screening and early detection of CRC. Here, CENPF is linked to colorectal carcinoma.