To confirm the expression pattern of VCAM1 involved in the progression of CRC, real-time PCR and Western blotting were performed in 8 cell lines, including FHC (normal colon mucosa), RKO, Caco-2, HCT15, SW620, LoVo, HCT116, and SW480, and the results showed obviously higher levels of VCAM1 mRNA and protein in human colorectal cancer cells than in normal colon mucosa cells (Figures 1A,B). The gene discussed is VCAM1; the disease is colorectal cancer.