Based on the new prediction model, the probability of HCC diagnosis could be calculated as follows: Prob (HCC) = 1/[1 + Exp(−XB)], where XB = −0.26−1.30 × RQ_ miR-92a-3p + 0.020 × Serum_AFP + 0.025 × RQ_ miR-21-5p. This evidence concerns the gene AFP and hepatocellular carcinoma.