Moreover, significantly reduced percentages of IFN-γ+ (45.0 ± 5.12 vs. 38.12 ± 8.82, p = 0.016; Figure 3C) and TNF-α+ (88.30 ± 2.97 vs. 78.74 ± 7.49, p = 0.011; Figure 3D) were also observed in CD226-blocked CD4+ T cells from PBC patients. This evidence concerns the gene TNF and primary biliary cholangitis.