Blocking CD226 on CD8+T cells from PBC patients resulted in significantly decreased frequencies of CD107a+ (81.12 ± 5.05 vs. 72.73 ± 7.75, p = 0.032; Figure 2D), IFN-γ+ (69.18 ± 6.73 vs. 52.86 ± 15.58, p = 0.024; Figure 2E), and TNF-α+ (76.42 ± 5.88 vs. 58.56 ± 14.71, p = 0.021; Figure 2F) cells among CD8+CD226+T cells, compared with non-blocked cells. This evidence concerns the gene TNF and primary biliary cholangitis.