With regard to the phenotypic analysis of CD4+T cells, the percentage of CD4+CD226+ T cells was significantly higher in PBC patients than in DCs (63.07 ± 13.30 vs. 52.55 ± 8.54, p < 0.001) and HCs (63.07 ± 13.30 vs. 50.10 ± 11.70, p < 0.001) (Figure 1C). This evidence concerns the gene CD4 and primary biliary cholangitis.