Stiffness of intact and decellularized segments (Figures 4Ai,Bi) increased with angII treatment in the first 4 weeks (intact H4 vs. intact NN, p < 0.0001; decellularized H4 vs. decellularized NN, p < 0.0001) and increased further in the next 4 weeks of hypertension (intact HH vs. intact H4, p < 0.01; decellularized HH vs. decellularized H4, p < 0.0001). The gene discussed is AGT; the disease is Hypertension.