To study the role of MAOA in the progression and development of NSCLC promoted by HPV-16 E7, CRISPR/Cas9 technique was used to knock out MAOA in HPV-16 E7-overexpressing NSCLC cells (16 E7) to construct stable NSCLC cell lines with HPV-16 E7 overexpression and MAOA knockout (16 E7-MAOA KO). This evidence concerns the gene MAOA and non-small cell lung carcinoma.