JAG2 and breast cancer: As we expected, the flow cytometric analysis showed that as compared with that of the control group (i.e., medium-treated UMUC3), the proliferation rate of these BC cells was significantly inhibited by Mel (2.0 mM) and furthermore significantly inhibited in KDNotch/JAG2 plus Mel treatment in time intervals from 0, 12, 48 to 72 h (Figure 6).