Twelve NSCLC cell lines (HCC827, H661, Calu-3, H1299, H460, H596, H358, H292, H1650, H1975, H1395, and A549) with the wild type ALK gene were selected according to Pharmacogenomics datasets of the Cancer Cell Line Encyclopedia (CCLE) project 27 and used in this study. The gene discussed is ALK; the disease is non-small cell lung carcinoma.