TGM2 and hepatocellular carcinoma: To further validate the proteomic results and to confirm the involvement of the potential candidate molecule with differential expressions in HCC cell EMT, western blotting comparative analysis was performed, and showed that TG2 was significantly upregulated during CAF-induced EMT in Hep3B, 97L, and Huh7 cell lines after treated with the CAF-CM for 48 h (Figure 4D and Figure S3).