SGTA and infection: Hexamethylene amiloride (HMA)— demonstrated to inhibit HCV p7, HIV-1 Vpu, and coronavirus E viroporin channel activity27,28—was found to specifically inhibit wt HAstV1 and HAstV4 infection, but not HAstV1-AUGm, -PTC1, or -5L mutant virus infection when used at noncytotoxic concentrations in Caco2 cells (5 μM; Fig. 6i and Supplementary Fig. 20).