MAP1LC3B and lung carcinoma: To reveal the potential mechanism by which hederagenin blocked the late autophagic flux, we also transfected lung cancer cells with the mCherry-GFP-LC3B tandem construct that can label autophagosomes with yellow fluorescence (overlap of mCherry+ and GFP+) and autolysosomes with red (quenching of GFP signal due to low pH inside lysosome).