To further confirm the effect of SB203580 (10 μM) and IL-6 (10 ng/mL) on PLum-AD and PLum-AI cells at the molecular and morphological levels, we performed immunofluorescence characterization and quantification of two EMT markers, Cytokeratin (CK)-8 (an epithelial luminal cell marker) and Vimentin (Vim) (a mesenchymal cell marker). Here, IL6 is linked to Alzheimer disease.