EGF and gastric cancer: Human gastric cancer cells SGC7901 were adherent growth cells, the culture condition was RPMI-1640 (10%FBS), CSC-G were suspension-growing cells, the culture condition was DMEM/F12 (1:1) serum-free culture medium containing EGF (20 ng/ml) and bFGF (20 ng/ml), and the adherent CSC-G were in the mesenchymal shape (Figure 1).