Importantly, we performed PCR-array in MYH9 knockdown ESCC cells and matched NC cells and together with the available TCGA database, we validated the associations among MYH9 and the significant changed genes of angiogenesis and epithelial-to-mesenchymal transition (EMT) pathways in ESCC and other squamous carcinomas. The gene discussed is MYH9; the disease is squamous cell carcinoma.