It was found that MYH9 was significantly positively correlated with angiogenesis markers FLT1, KDR, TEK, and VEGFC, significantly positively correlated with EMT markers SNAI2, KRT14 and CDH2. The above-mentioned data suggest a possible mechanism of action of MYH9. Combined with our results, it is suggested that MYH9, as a low-frequency mutant gene in ESCC, can regulate the metastasis of ESCC through angiogenesis and EMT pathway. This evidence concerns the gene VEGFC and esophageal squamous cell carcinoma.