To facilitate cell adhesion and migration inside the microfluidic platform, the T-cell and cancer-cell channels of the platform were first incubated with a solution of 5 μg/mL intercellular adhesion molecule 1 (ICAM-1, R&D System) and 25 μg/mL fibronectin (Sigma-Aldrich) for 1 h at 37 °C, respectively, followed by flushing with phosphate buffer saline (PBS). Here, ICAM1 is linked to cancer.