Many of these pathways were similar to those identified by KEGG and GO analysis, including “adaptive immune system,” “extracellular matrix organization,” “axon guidance,” and “demyelinating disease.” Interestingly, DEGs were highly involved in neuronal and glial function as well as myelination according to DO, such as insulin-like growth factor I (IGF-I) (Additional file 2: Tables S6-7) and the antioxidant superoxide dismutase 2 (SOD2) (Additional file 2: Table S7) [35, 36]. Here, IGF1 is linked to demyelinating disease.