Our study confirms the good diagnostic performances of WB bone SPECT/CT in prostate BR with sensitivity, specificity, positive and negative predictive values of 86.7% [69.3–96.2], 98.8% [93.6–100.0], 96.3% [78.7–99.5], and 95.5% [89.4–98.1] when equivocal WB bone SPECT/CT were considered as negative, in comparison to the BVC and regardless of the PSA level. The gene discussed is KLK3; the disease is male reproductive organ cancer.