To improve the performance of this panel, we plan to remove or improve underperforming amplicons which take up sequencing reads, such as those covering TERT promoter (discussed below), DDX3X and EIF1AX. Both DDX3X and EIF1AX mutations account for 10% of melanomas and they generally co-occur with NRAS mutations [27,28]. This evidence concerns the gene NRAS and melanoma.