The effect of SAM to decrease methylation was proved to be beneficial in certain genes (HT-29: DTX1 [31], GATA4 [32], SEZ6L [33], TP53INP1 [34]; SW480: HAAO [35], TAL1 [36]), whose hypermethylation was associated with tumor progression according to the scientific literature (Figure 3c). Here, GATA4 is linked to neoplasm.