GLI1 and cancer: The SMO gene exhibited the highest level of mRNA expression and the greatest variation between cell lines (22.98 ± 31.80, 95% CI 11.70~31.25), compared with PTCH1 (8.72 ± 10.44, 95% CI 5.02~12.42), GLI2 (1.25 ± 2.08, 95% CI 0.56~1.99) and GLI3 (18.04 ± 20.26, 95% CI 10.85~25.22), whereas GLI1 (0.17 ± 0.25, 95% CI 0.08~0.26) that functions as an amplifier of HH signal, consistently showed low expression in all cancer cell lines.