To investigate the effect of IKKε activation on metabolism, we used two cellular model systems: (i) doxycycline‐inducible Flp‐In 293 HA‐IKKε‐expressing cells and their respective GFP‐expressing controls (Flp‐In 293 HA‐GFP cells) and (ii) two breast cancer cell lines, T47D and MDA‐MB‐468, where the kinase was silenced via siRNA. This evidence concerns the gene IKBKE and breast cancer.