When the cut-off values of the γ-GT/ALT ratio and AFP and PIVKA-II levels were set as 1.580, 499.80 ng/mL, and 157.39 mAu/mL, respectively, in patients with HCC who were positive for HBV DNA, there were no significant differences in AUROCs between the γ-GT/ALT ratio and AFP (P = 0.069). This evidence concerns the gene GPT and hepatocellular carcinoma.