When the γ-GT/ALT ratio, AFP, and PIVKA-II were used in combination for the diagnosis of early-stage HCC, HCC, HCC with HBV DNA+, and HCC with HBV DNA−, the diagnostic performance of the γ-GT/ALT ratio combined with AFP or PIVKA-II was better than that of the biomarkers alone. The gene discussed is AFP; the disease is hepatocellular carcinoma.