The AUROCs of PIVKA-II combined with γ-GT/ALT ratio for the diagnosis of early-stage HCC, HCC, HCC with HBV DNA+, and HCC with HBV DNA− were higher than those of PIVKA-II combined with AFP and AFP combined with the γ-GT/ALT ratio, implying improvement of the diagnostic value. This evidence concerns the gene AFP and hepatocellular carcinoma.