Compared with the results in patients with early-stage HCC, the diagnostic value of AFP and PIVKA-II increased in patients with advanced HCC (P = 0.002, P = 0.010); however, there were no significant differences in the diagnostic value of the γ-GT/ALT ratio between early-stage HCC and advanced HCC (P = 0.072). This evidence concerns the gene GPT and hepatocellular carcinoma.