To further analyze the role of human and mouse SR-B1, we took advantage of the fact that the murine hepatoma Hepa1-6 cells do not express SR-B14 and generated a variant cell line deficient for murine CD81 (CD81 knockout (KO) Hepa1-6 or CD81KOH16) using the CRISPR-Cas9 system. This evidence concerns the gene CD81 and hepatocellular carcinoma.