Based on variant allele frequencies, they revealed a founding clone in JAK2V617F or U2AF1 and three subclones with the MYB subclone developing parallel to the nested subclones harboring ASXL1/HCFC1 and RUNX1/IDH1, the latter expanding during transformation to AML [123]. This evidence concerns the gene IDH1 and acute myeloid leukemia.