Here, based on T cell immunoglobulin and mucin domain containing 4 (Tim-4) expression, we identified 2 phenotypically, transcriptionally, ontogenically, metabolically, and functionally distinct TAM subsets, Tim-4+ and Tim-4– TAMs, in an ovarian cancer peritoneal metastasis model, then extended our studies to patients with ovarian cancer. The gene discussed is TIMD4; the disease is ovarian cancer.