Then we detected the expression of the key proteins in the xenograft tumor, and found that the combined treatment of irradiation and the maximum noncytotoxic dose of tetrandrine could also inhibit the upregulation of p‐CDC25C, p‐CDK1 and p‐ERK, and p‐MEK caused by irradiation, increase the expression of cyclinB1, which is consistent with that in vitro. Here, CDC25C is linked to neoplasm.