In both US28-expressing NIH-3T3 fibroblasts and U251 glioma cells, an increased phosphorylation of tyrosine 105 of PKM2 and a corresponding increase of PKM2 dimer compared with mock cells was observed, and this effect was mediated by HIF-1 [29] (Figure 2). The gene discussed is HIF1A; the disease is central nervous system cancer.