Silencing GATA5 expression in HLEcells (accompanied with treatment by Paclitaxel) stimulated expression of the reprogramminggenes, while enhancing expression of GATA5 in the Bel7402 and PLC/ PRF/5 cells (accompaniedwith treatment by Paclitaxel) could inhibit expression of the reprogramming genes, such asNanog, c-Myc and Sox2. The results indicated that GATA5played a role in promoting Paclitaxel to inhibit expression of the reprogramming genes inHCC cells, which enhanced sensitivity of the HCC cells to Paclitaxel, and also inhibitingcancer stem cell formation and cancer cells aggressiveness. The gene discussed is MYC; the disease is hepatocellular carcinoma.