The results displayed that overexpression of GATA5 stimulatesPaclitaxel effect to decrease expression of the reprogramming genes Nanog, EpCAM,c-Myc, Sox2 and two stemness marker (CD44 and CD133) in the HCC cells invitro. Our results revealed that GATA5 played an important rolein Paclitaxel inhibiting the malignant behaviors of HCC cells by blocking expression of thereprogramming related genes and stemness markers. The gene discussed is CD44; the disease is hepatocellular carcinoma.