The results of this study revealed the following: miR‐20a‐5p regulates RGMa and RGMa regulates RhoA—namely, miR‐20a‐5p, RGMa and RhoA coexist as a pathway; the miR‐20a‐5p‐RGMa‐RhoA pathway controls neuronal morphology in primary hippocampal neurons; silencing miR‐20a‐5p delays epileptogenesis through RGMa‐RhoA‐mediated synaptic plasticity but did not change MFS in the PTZ‐induced epilepsy rat model. This evidence concerns the gene RGMA and epilepsy.