Although not in sequential samples, but in GBM and control brain comparisons, a differential methylation of Wnt genes (e.g., Wnt2, Fzd6) and pathways, and both up- (Wnt5a, Fzd7, Fzd5) and downregulation of Wnt pathway transcripts (Wnt10b, Wnt7a, Wnt7b, Wnt2b) have been noted (Etcheverry et al. 2010; Nagarajan et al. 2014). This evidence concerns the gene WNT5A and glioblastoma.