Enrichment analyses highlighted regions representing hypomethylated binding sites for transcription factors (e.g., ESR1, ESR2, CTCF, RUNX1) and histone proteins (e.g., H3K27me3; H3K4me1; H3K4me3) likely relevant to CSC differentiation and GBM development (Klughammer et al. 2018; Huang et al. 2004; Bernstein et al. 2006; Hyun et al. 2017). This evidence concerns the gene ESR1 and glioblastoma.