Our findings revealed key information on the signaling pathways by which EcN might exert its pro-apoptotic impacts on the colon cancer cells through up-regulation of PTEN and Bax and down-regulation of AKT1. Moreover, similar to other probiotic strains, this bacterium might affect other cancers by modulating different signaling pathways. This evidence concerns the gene AKT1 and colonic neoplasm.