ANXA5 and hepatocellular carcinoma: Cells in mid-apoptosis stain with both PI and labeled Annexin V. Dot-plot analysis revealed the percentage of double-staining cells for each treatment group: 19%, 43% 37% and 90% for untreated, cytokines, MK-2206 and both (SKBR3), 15%, 22.2%, 12.4% and 84.1%, respectively for MDA-MB-468; 11.6%, 17.4%, 22% and 69.9% respectively for MDA-MB-453 cells and 6.1%, 20.2%, 10.3% and 45.5%, respectively, for HCC-1419 (Figure 4).