In summary, our study is the first demonstration of the fact that AVME, a prenylated flavanone isolated from the African medicinal plant E. droogmansiana, induces cytotoxic effect in breast cancer cell lines by triggering apoptosis via ROS-mediated mitochondrial pathway characterized by activation of caspase-3 and caspase-9 and downregulation of Bcl-2 and Bcl-XL proteins. Here, CASP3 is linked to breast cancer.