Taken together, our findings reveal that mating induced steroid hormone release, which signals to adjacent intestinal stem cells, controls their proliferation and, more importantly, differentiation of committed precursor cells through Eip75B/PPARγ. Ecdysone control of cell fate ensures the production of absorptive enterocytes during mating related intestinal adaptations and induces enterocyte fate in a Drosophila intestinal tumor model marked by loss of enterocyte differentiation. This evidence concerns the gene PPARG and intestinal neoplasm.