Furthermore, we found 14 specific pathways with statisical significance (Table 9) contributing to GBM and corresponding 17 genes, SLC7A5, mLST8, Lipin-1, Tel2, CLIP-170, ATG1, SLC3A2, RNF152, eIF4B, GATOR1, STRAD, IGFR, IRS1, PDK1, TSC1/6, Rheb. These genes have also been verified in many studies. The gene discussed is IRS1; the disease is glioblastoma.