Consistently with previous reports32,35, MCF10A cells exhibited normal acinar morphogenesis in 3D cultures, while ectopic expression of mut-p53 variants R175H or R280K, but not wt-p53 silencing per se36, inhibited luminal clearance, reminiscent of the filled lumen phenotype of ductal carcinoma in situ (Supplementary Fig. 2a). The gene discussed is TP53; the disease is ductal breast carcinoma in situ.