To interrogate the in vivo role of endothelial IP3R1 in regulating atherosclerosis, we established a conditional endothelial-specific IP3R1 depletion mouse by crossing IP3R1fl/fl mice to ApoE−/− mice followed by breeding of offspring with iCDH5-Cre transgenic mice (Supplementary Fig. 11a-c)39. The gene discussed is APOE; the disease is atherosclerosis.