To understand the in vivo role of epsins in facilitating atherosclerosis through endothelial cell dysfunction, we employed the ApoE−/− atherosclerotic mice with an endothelial cell-specific deletion of epsin 1 on a global epsin 2 knock-out background (EC-iDKO/ApoE−/−) (Supplementary Fig. 1a-c)18,19,25. This evidence concerns the gene EPN1 and atherosclerosis.